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Bacteriophage and Biodosimetry
As Ultra Violet Light
(UV) technology gains importance in
the United States as a means to
disinfect water and wastewater, the
use of Bacteriophage as a biological
indicator of UV Dose is gaining
equal prominence. Authorities are
relying on data generated by BioVir,
from pilot and plant scale
site-specific biodosimetry studies,
to make regulatory decisions.
Why Biodosimetry?
Although the energy put out by UV
lamps used in a given treatment
configuration can be determined by
physical measurements there are a
variety of variables that make the
overall dose of UV (mJ/Cm2
) difficult to measure. Unknown
hydraulic parameters, for example,
can have a profound affect on the
amount of UV Light the microbes in
the water being treated receive.
Biodosimetry, using MS-2 coliphage,
is being relied upon to provide
reliable information regarding the
overall UV dose and subsequent log
reduction of organisms throughout
the UV treatment process.
During a typical study BioVir
prepares high concentrations MS-2
coliphage and determines their
sensitivity to UV using a
collimating beam device. The
coliphage is then shipped to the
treatment facility and kept cold
until use. The UV treatment system
to be evaluated is run as per
standard operating procedures and
the coliphage added by utility
personnel to the influent stream at
a target concentration of typically
105 to 106/mL.
The concentration of MS-2 in the
influent and in the product water is
measured and their degree of
reduction is correlated to the UV
dose (as determined from the
collimated beam sensitivity check)
that would be required to cause such
a reduction in numbers.
BioVir Laboratories has been
involved in numerous biodosimetry
exercises in both drinking water and
wastewater treatment systems. Our
experienced, knowledgeable staff is
critical to efficiently and
accurately process the large number
of samples produced during each
short duration challenge event.
Please contact us to discuss your
study requirements.
Bacteriophage
NWRI UV Guidelines
EPA
Method 1601/1602
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